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PeptideBasics101

peptide

SS-31 (elamipretide)

A four-amino-acid peptide that binds cardiolipin in the inner mitochondrial membrane; granted FDA accelerated approval as Forzinity in September 2025 to improve muscle strength in Barth syndrome, while trials in primary mitochondrial myopathy and dry macular degeneration have missed or not yet reported their primary endpoints.

Data refreshed 2026-09-23 · Based on 8 published references

Names

Generic: elamipretide

Brands: Forzinity

Also called: SS-31, MTP-131, Bendavia, Ocuvia, Szeto-Schiller peptide 31, elamipretide hydrochloride

Regulatory (US)

FDA approval: approved

503A compounding: restricted

Forzinity — Improve muscle strength in adults and children with Barth syndrome weighing at least 30 kg (accelerated approval) (US, 2025)

Molecule

Formula: C32H49N9O5

MW: 639.8 g/mol (free base); 749.2 g/mol as the trihydrochloride in Forzinity

CAS: 736992-21-5

PubChem entry

Sequence: D-Arg-Dmt-Lys-Phe-NH2 (Dmt is 2,6-dimethyl-L-tyrosine; C-terminal amide)

Origin

Discovered by: Hazel H. Szeto (Weill Cornell Medical College) and Peter W. Schiller (Clinical Research Institute of Montreal)

Year: 2004

Developer: Stealth BioTherapeutics

What it is

SS-31, now called elamipretide, is a very small lab-made peptide: four amino acids long. It was designed to slip into mitochondria, the structures inside cells that make energy, and to stick to a fat molecule called cardiolipin that sits in their inner wall. Cardiolipin helps hold the energy-making machinery together, and it is faulty in a rare inherited disease called Barth syndrome.

In September 2025 the FDA approved elamipretide under the brand name Forzinity, as a once-daily injection to improve muscle strength in people with Barth syndrome who weigh at least 30 kg. The approval is an “accelerated” one, meaning it rests on an early signal and must be confirmed by a further trial. It is the only approved use.

Elamipretide is the tetrapeptide D-Arg-Dmt-Lys-Phe-NH2 (Dmt, 2,6-dimethyltyrosine; C32H49N9O5, 639.8 g/mol), one of the Szeto-Schiller peptides. Its alternating aromatic and cationic residues give a net +3 charge at physiological pH; the SS peptides are cell-permeable and were reported to concentrate about 1000-fold in the inner mitochondrial membrane (Zhao et al., J Biol Chem 2004). It binds cardiolipin through electrostatic and hydrophobic interactions; the label describes it as a mitochondrial cardiolipin binder that improves mitochondrial morphology and function. Preclinical work proposes that binding prevents cardiolipin from converting cytochrome c into a peroxidase.

Label pharmacokinetics: after subcutaneous injection, peak concentrations occur at 0.5–1 hour and absolute bioavailability is about 92%. Volume of distribution is about 0.5 L/kg and plasma protein binding about 39%. Elamipretide is degraded from the C-terminus to inactive tri- and dipeptide metabolites, and about 100% of a dose is recovered in urine within 48 hours. Exposure rises with renal impairment, and the label halves the dose when eGFR is under 30 mL/min without dialysis.

Who made it and when

The peptide came out of a long collaboration between Hazel Szeto, a pharmacologist at Weill Cornell Medical College in New York, and Peter Schiller, a peptide chemist in Montreal; “SS” stands for Szeto-Schiller. Their family of small peptides was built from alternating ring-shaped and positively charged amino acids, and some of them turned out to gather inside mitochondria. The key paper describing these peptides as mitochondria-targeted compounds appeared in 2004.

A Massachusetts company, Stealth BioTherapeutics, took the compound into human trials under the names MTP-131 and Bendavia, and later elamipretide. After trials in heart and kidney conditions, the company focused on rare mitochondrial diseases and on a form of age-related vision loss.

The Szeto-Schiller peptides were developed jointly at Weill Cornell and the Clinical Research Institute of Montreal. Zhao et al. (J Biol Chem 2004) reported that these cell-permeable peptides, built on alternating aromatic and basic residues with dimethyltyrosine, localise to the inner mitochondrial membrane, reduce mitochondrial ROS, inhibit permeability transition and swelling, and improved contractile force in an ex-vivo reperfused heart. Szeto’s 2014 review in the British Journal of Pharmacology describes SS-31 as the first cardiolipin-protective compound. The early US patent covering the peptides’ use to prevent mitochondrial permeability transition (US 7,576,061; Cornell Research Foundation and IRCM; priority 2003) is one of six US patents listed on the Forzinity label; Google Patents gives its expiry as 2026-01-20.

Stealth BioTherapeutics ran studies in reperfusion injury during coronary intervention (EMBRACE STEMI) and renal-artery angioplasty (EVOLVE), heart failure and skeletal muscle function in older adults before concentrating on primary mitochondrial myopathy, Barth syndrome and dry age-related macular degeneration. The Barth syndrome NDA (215244) was submitted on 2024-01-29, received a complete response on 2025-05-15, and was approved under the accelerated pathway on 2025-09-19, with a rare pediatric disease priority review voucher.

What the data say

The approval rests on very few people. Barth syndrome is ultra-rare, and the key trial had 12 patients. In the blinded part, where patients swapped between the drug and a dummy injection for 12 weeks each, the drug did not beat placebo on its two main goals, walking distance and fatigue. Leg-muscle strength rose later, during several years of open-label treatment when everyone knew they were on the drug. That later gain is what the FDA accepted as an early sign of effect.

Elsewhere the record is mixed. A large trial in adults with inherited mitochondrial muscle disease was negative. A vision trial in dry macular degeneration missed its main goals, and a larger one is still running.

TAZPOWER (NCT03098797): 12 males with Barth syndrome received 40 mg subcutaneously daily in a 12-week crossover. Neither primary endpoint (6MWT, BTHS-SA total fatigue) was met. In the open-label extension, 6MWT improved by 95.9 m at 36 weeks (p = 0.024), and the label reports median knee extensor strength gains of 34 to 68 newtons from weeks 12 to 168 against a baseline of 124 N, as descriptive statistics without a control group.

MMPOWER-3 (NCT03323749; n=218): at 24 weeks the least-squares 6MWT difference was −3.2 m (p = 0.69) and PMMSA fatigue did not differ. NuPOWER (NCT05162768; n=102, 60 mg daily, nuclear-DNA disease) has no posted results.

ReCLAIM-2 (NCT03891875; n=176): no significant effect on low-luminance visual acuity or geographic atrophy growth at 48 weeks; ellipsoid-zone loss progressed about 43% more slowly (nominal p = 0.0034). ReNEW (NCT06373731; n=313) has as its registered primary endpoint the week-48 rate of change in the macular area of photoreceptor loss, the ellipsoid-zone measure that Ehlers et al. said would serve as the Phase 3 primary endpoint.

Safety: injection-site reactions occurred in all treated Barth patients; hypersensitivity and eosinophilia are labeled.

Regulatory picture

Elamipretide is FDA approved, but only for one narrow use: improving muscle strength in children and adults with Barth syndrome who weigh at least 30 kg. The approval is conditional. The FDA first turned the application down in May 2025, then granted accelerated approval in September 2025 based on the muscle-strength data, and required the company to run a new placebo-controlled trial. If that trial fails to confirm a real effect, the FDA can withdraw the approval. The confirmatory trial began enrolling in 2026.

It is not approved for mitochondrial myopathy, macular degeneration or anything else. Because an approved product exists, pharmacies may not make copies of it regularly or in large amounts unless it is on the FDA’s drug shortage list.

Approval: Forzinity (elamipretide) injection, NDA 215244, accelerated approval under section 506(c) and 21 CFR 314.510 on 2025-09-19 for adult and pediatric Barth syndrome patients weighing at least 30 kg, based on knee extensor strength as an intermediate clinical endpoint. The postmarketing requirement is a randomized, double-blind, placebo-controlled trial, with study completion scheduled for 09/2029; 4TAZPower (NCT07531251, Phase 3b/4, ages 5–55) began in July 2026. The labeled dosage is 40 mg subcutaneously once daily, or 20 mg with eGFR under 30 mL/min not on dialysis.

Compounding: elamipretide is not on the 503A bulks list or in any 503A nomination category. Because it is the active ingredient of an approved drug, section 503A bars compounding drugs that are essentially copies of it regularly or in inordinate amounts while it is commercially available (a product on FDA’s drug shortage list does not count as commercially available), so this article records it as restricted.

Other programmes: none of the other indications has been filed or approved. No approval outside the US was verified for this article.

Doses reported in trials

Doses reported in studies, exactly as the cited trial reported them. They are not personal dosing instructions. Population, route and schedule matter more than the number.

TrialPhaseStatusPopulationDoseResult
TAZPOWER
NCT03098797
2/3Completed12 males aged 12 and over with genetically confirmed Barth syndrome40 mg subcutaneous once daily; 12-week crossover versus placebo with a 4-week washout, then open-label extensionNeither primary endpoint (6-minute walk, BTHS-SA fatigue) was met in the crossover; at 36 weeks of the open-label extension, 6-minute walk improved by 95.9 m (p = 0.024) (Reid Thompson et al., Genet Med 2021)
MMPOWER-3
NCT03323749
3Terminated after the double-blind part missed its primary endpoints218 adults aged 16–80 with genetically confirmed primary mitochondrial myopathy40 mg subcutaneous once daily for 24 weeks versus placebo6-minute walk difference −3.2 m (95% CI −18.7 to 12.3; p = 0.69); no difference in PMMSA total fatigue (Karaa et al., Neurology 2023)
NuPOWER
NCT05162768
3Completed December 2024; no results posted on the registry102 adults aged 18–70 with primary mitochondrial disease due to nuclear DNA variants60 mg subcutaneous once daily for 48 weeks versus placeboNo peer-reviewed report found; Stealth presented overall outcomes and a POLG1-subgroup analysis at Euromit in 2026
ReCLAIM-2
NCT03891875
2Completed176 adults aged 55 and over with dry age-related macular degeneration and non-central geographic atrophy40 mg subcutaneous once daily for 48 weeks versus placebo (randomised 2 to 1)Missed both primary endpoints (low-luminance visual acuity, geographic atrophy growth); slower ellipsoid-zone loss on predefined secondary measures, nominal p values only (Ehlers et al., Ophthalmol Sci 2025)
ReNEW
NCT06373731
3Active, not recruiting (completion estimated September 2027)313 adults aged 55 and over with dry age-related macular degeneration40 mg subcutaneous once daily for 96 weeks versus placebo—
4TAZPower (confirmatory)
NCT07531251
4Recruiting (started July 2026)48 males aged 5–55 with genetically confirmed Barth syndrome, plannedSubcutaneous elamipretide (80 mg/mL solution) once daily versus matching placebo; the registry does not state the milligram dose—

Enforcement history

Reported side effects

Interactions

Contraindications

Storage

Unopened: Forzinity vials are labeled for refrigeration at 2–8 °C (36–46 °F) and labeled not to be frozen.

Reconstituted / in use: Not applicable; Forzinity is a ready-to-use solution. The label states that an opened vial may be kept refrigerated or at 20–25 °C and is discarded 8 days after first opening.

Product characteristics

Form: Ready-to-use aqueous solution for subcutaneous injection, 280 mg/3.5 mL (80 mg/mL) single-patient-use vials, preserved with benzyl alcohol

Appearance: Clear, colorless to yellow solution

Solubility: The hydrochloride salt is freely soluble in water (label)

Stability: FDA's approval letter sets expiry dating at 48 months from manufacture when stored at 2–8 °C

Compound information

Synthetic tetrapeptide containing one D-amino acid (D-arginine) and the non-natural residue 2,6-dimethyltyrosine, formulated as the trihydrochloride salt in the approved product.

Patents

References

This page summarises the published sources below. PeptideBasics101 does no original research. Trial doses and results are reported as the cited study or label reported them. Sources can themselves be wrong or superseded; if you find a statement here that does not match its source, please tell us through our corrections process. Sources were last checked on 2026-09-23.

  1. Forzinity (elamipretide) prescribing information (FDA label, 2025) (accessdata.fda.gov)
  2. FDA accelerated approval letter, NDA 215244 (September 2025) (accessdata.fda.gov)
  3. FDA Drug Trials Snapshots: Forzinity (fda.gov)
  4. Szeto HH. First-in-class cardiolipin-protective compound as a therapeutic agent to restore mitochondrial bioenergetics. Br J Pharmacol 2014 (pubmed.ncbi.nlm.nih.gov)
  5. Reid Thompson W et al. A phase 2/3 randomized clinical trial followed by an open-label extension of elamipretide in Barth syndrome (TAZPOWER). Genet Med 2021 (pubmed.ncbi.nlm.nih.gov)
  6. Karaa A et al. Efficacy and safety of elamipretide in individuals with primary mitochondrial myopathy (MMPOWER-3). Neurology 2023 (pubmed.ncbi.nlm.nih.gov)
  7. Ehlers JP et al. ReCLAIM-2, a randomized phase II trial of elamipretide in age-related macular degeneration with geographic atrophy. Ophthalmol Sci 2025 (pubmed.ncbi.nlm.nih.gov)
  8. PubChem: elamipretide (CID 11764719) (pubchem.ncbi.nlm.nih.gov)

Clinical trial entries in the table above link to their ClinicalTrials.gov registry records. Spotted an error? See corrections.

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